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产地 | 国产/进口 |
品牌 | YLKbio |
货号 | YLKsj0079 |
用途 | 科研 |
包装规格 | 150u/450u/1ku |
纯度 | 详询% |
CAS编号 | 9028-79-9 |
是否进口 | 否 |
中文名称:半乳糖氧化酶
英文名:
Galactose oxidase
含量:
高纯,30u/mg
CAS号:
9028-79-9
Method
The reaction velocity is measured in a peroxidase/o-tolidine coupled system as an increase in A425 resulting from the oxidation of galactose. One unit results in a change in A425 of 1.0 per minute at 25°C and pH 6.0 under the defined conditions.
Reagents
1)0.1 M Potassium phosphate buffer, pH 6.0
2)0.5% o-tolidine. Note: o-tolidine has been reported to be carcinogenic. Handle with care. 3)Peroxidase. Dissolve at a concentration of approximately 60 u/ml in reagent grade water. 4)10% galactose. Allow to come to equilibrium of mutarotation by allowing to stand overnight.