中文名：磷酸葡萄糖异构酶

英文名：
Phosphoglucose isomerase
别名：
CAS号：
9001-41-6

REAGENTS:

A. 250 mM Glycylglycine Buffer, pH 7.4 at 25E

(Prepare 100 ml in deionized water using Glycylglycine, Free Base,. Adjust the pH to 7.4 with 1 M NaOH.)
B. 100 mM D-Fructose 6-Phosphate Solution (F-6-P)

(Prepare 1 ml in deionized water using D-Fructose 6-Phosphate, Disodium.)

C. 20 mM β-Nicotinamide Adenine Dinucleotide Phosphate Solution (NADP) (Prepare 1 ml in deionized water using β-Nicotinamide Adenine Dinucleotide Phosphate,
Sodium Salt. PREPARE FRESH.)

D. 100 mM Magnesium Chloride Solution (MgCl2)

(Prepare 10 ml in deionized water using Magnesium Chloride, Hexahydrate,.)

E. Glucose-6-Phosphate Dehydrogenase Enzyme Solution (G-6-PDH)
(Immediately before use, prepare a solution containing 50 units/ml of Glucose-6-Phosphate Dehydrogenase, in cold deionized water.)
F. Phosphoglucose Isomerase Enzyme Solution (PGI) (Immediately before use, prepare a solution containing 0.3 - 0.7 unit/ml in cold deionized water.)