中文名：脱氧核糖核酸酶Ⅱ

英文名：
DNase II
别名：
去氧细胞核酸酶;氧核糖核酸-5ˊ-寡核苷酸水解酶;脱氧核糖核酸去聚合酶
CAS号：
9025-64-3
Method
That developed by Kunitz (1950) based upon the increase in absorbance at 260 nm during the hydrolysis of DNA. The method has been adapted from that used for DNase I by reducing the pH to 4.6 and lowering the magnesium concentration to 0.001 M. One unit causes an increase in absorbance at 260 nm of 0.001 per minute per ml at 25°C and pH 4.6 under specified conditions. Bernardi (1971) discusses the relationship of absorbance units so defined to terminal phosphate formation, i.e., substrate bonds hydrolyzed.

介绍：活力定义： One Unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25°C, pH 4.6 using highly polymerized DNA as substrate.
分子量：38.0 kDa
最适pH：4.5-5.0
等电点：8.1
储存条件： -20℃
用途： DNase II has been used to treat transformed cells during the purification of β-lactamase. It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma c
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