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产地 | 国产/进口 |
品牌 | YLKbio |
货号 | YLKsj0039 |
用途 | 科研 |
包装规格 | 5u/25u |
纯度 | 详询% |
CAS编号 | 9025-35-8 |
是否进口 | 否 |
中文名:α-半乳糖苷酶
英文名:
α-Galactosidase preparation from green coffee beans
别名:
α-D-半乳糖苷半乳糖水解酶
CAS号:
9025-35-8
REAGENTS:
A. 100 mM Potassium Phosphate Monobasic Solution
(Prepare 100 ml in deionized water using Potassium Phosphate, Monobasic, Anhydrous.)
B. 100 mM Potassium Phosphate Dibasic Solution
(Prepare 100 ml in deionized water using Potassium Phosphate, Dibasic, Trihydrate.)
C. 100 mM Potassium Phosphate Buffer, pH 6.5 at 25?C
(Prepare 100 ml by adjusting 50 ml of Reagent A to pH 6.5 at 25?C by adding Reagent B.)
D. 9.9 mM p-Nitrophenyl ?-D-Galactopyranoside Solution (PNP-Gal)
(Prepare 4 ml in deionized water using p-Nitrophenyl ?-D-Galactopyranoside.)
E. 200 mM Borate Buffer, pH 9.8 at 25?C
(Prepare 100 ml in deionized water using Boric Acid, Sigma Prod. No. B-0252. Adjust to pH 9.8 at 25?C with 1 M NaOH.)
F. ?-Galactosidase Enzyme Solution
(Immediately before use, prepare a solution containing 0.05 - 0.10 units/ml of ?-Galactosidase in cold Reagent C.)